![]() Control of development by ionic currents. The contribution of sialic acid to the surface charge of the erythrocyte. EYLAR EH, MADOFF MA, BRODY OV, ONCLEY JL.Adsorption of monovalent cations to bilayer membranes containing negative phospholipids. Eisenberg M, Gresalfi T, Riccio T, McLaughlin S.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. Addition of the negatively charged lipid monosialoganglioside (GM1), on the other hand, enhances the accumulation of Con A receptors at the cathodal side of the cell. Our experimental results demonstrate that concanavalin A (Con A) receptors on embryonic muscle cells normally accumulate at the cathodal side of the cell, but that they can be induced to accumulate at the anodal side of the cell by preincubating the myotubes either with neuraminidase, a treatment that removes negatively charged sialic acid residues, or with the lipid diI, a treatment that adds positive charges to the surface of the cell. Conversely, if zeta 2 is less negative than zeta 1, the negatively charged macromolecule will accumulate at the anodal side of the cell. ![]() Our analysis of a simple model of the cell surface indicates that a negatively charged mobile macromolecule will be swept by this electro-osmotic flow of fluid to the cathodal side of the cell if its zeta potential, zeta 1, is less negative than the zeta potential of the cell surface, zeta 2. The imposition of an electric field parallel to the surface of the cell should produce, therefore, an electro-osmotic flow of fluid towards the cathodal side of the cell. The surfaces of most cells bear a net negative charge.
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